Protein

UniProt accession

A0A2K9VN69 [UniProt]

Protein name

Baseplate central spike protein

PhaLP type

endolysin

evidence: UniProt function annotation

probability: 99 % (predicted by ML model)

Protein sequence

MLMFNNSVKWFTGIVENREDPLMLGRVQVRIHGIHPSLSAHGDIQGLPTEDLLWITPVQDITSAAISGIGQSPTGIVEGSMVFGLALDERYLNCVILGTIAGQYTEKPLTTEGFCDPSGAYPRYIGNDVNILARGGINPETGQTTKPDSVYIRDENTGVAVNPDDEPLDQIPVDDNPDFTIEKMLRGDEGYREKWYLDSEGYPTIGIGHLIIYKKTSDLGIINNELSKLVGREVTNGRLTAEEVSKVFADDIEKTRRDMRKHPRIAPVYNKCNASRRMALENMAFQMGVGGLGKFKNSLAAMLAEEWKQAYDGLRQSVWANQTPGRSSRVSKIILTGNLESYGVIAPKKEESISDDPRARLRNARIAAYKTQRAEEDPEAPFTPQDTRIMFKEPKSSYSARYPYNHVYESESGHIIEIDDTPSHERYHRKHPSGTFEETRPDGTRVEKIEGDDYLIVKQGRKVNVKGNLQVVVEGDAQVYYMGNVMQTVDGNVTEFIRGNVNQTVEGTANMHVVGDVTAQLDANLTANVKGNAELMIEGNTTETIKGNYDLTVEGNFNMTVNGTKSDQVNGDWSRNCGASVKDIASGTVKIDGSRIDLG

Physico‐chemical properties

protein length:	599 AA
molecular weight:	66249,00000 Da
isoelectric point:	5,19062
aromaticity:	0,06845
hydropathy:	-0,52922

Domains

Domains [InterPro]

IPR023346

145–347

IPR023346 IPR010609

IPR052619

180–341

IPR052619 IPR009590

IPR023347

179–345

IPR023347

IPR002196

189–320

IPR002196

IPR001165

319–340

IPR001165

IPR046397

3–599

IPR046397

Protein sequence: A0A2K9VN69

1 599

Legend: Pfam SMART CDD TIGRFAM HAMAP SUPFAM PRINTS Gene3D PANTHER Other

Domains [InterPro]

InterPro ID	Start	End	Source	Name
IPR023346	145	347	SUPFAM	Lysozyme-like domain superfamily
IPR052619	180	341	PANTHER	Bacteriophage lysozyme-like
IPR010609	475	497	Pfam	Gp5, C-terminal
IPR023347	179	345	Gene3D	Lysozyme domain superfamily
IPR002196	189	320	Pfam	Glycoside hydrolase, family 24
IPR001165	319	340	PRINTS	T4-type lysozyme
IPR009590	33	178	Pfam	Protein Gp5, N-terminal OB-fold domain
IPR046397	3	599	HAMAP	Pre-baseplate central spike protein Gp5

Taxonomy

	Name	Taxonomy ID	Lineage
Phage	Shigella phage Sf20 [NCBI]	2024307	Straboviridae > Krischvirus > Krischvirus RB49
Host	No host information

Coding sequence (CDS)

Genbank protein accession

AUV63823.1 [NCBI]

Genbank nucleotide accession

MF327006 [NCBI]

CDS location

range 1929 -> 3728
strand +

CDS

ATGCTAATGTTTAATAACTCCGTTAAATGGTTTACCGGAATTGTAGAAAACCGGGAAGACCCATTAATGCTGGGGCGTGTTCAGGTTAGGATTCATGGTATACATCCCTCGCTATCCGCACACGGTGACATTCAAGGTCTTCCAACCGAAGATCTATTATGGATTACGCCAGTACAAGATATTACAAGCGCGGCGATCTCCGGTATAGGTCAAAGCCCTACTGGTATTGTTGAAGGTTCAATGGTATTTGGTTTGGCTTTAGATGAACGTTATTTAAACTGCGTTATTCTTGGTACTATTGCCGGACAATATACAGAGAAACCACTCACTACAGAGGGTTTTTGCGACCCTTCTGGTGCATATCCTCGTTATATTGGTAATGATGTTAATATTCTTGCTCGTGGCGGTATTAACCCGGAAACCGGACAAACAACAAAACCTGATAGTGTCTATATTCGAGATGAAAACACTGGAGTTGCAGTAAATCCAGACGACGAGCCATTAGACCAAATTCCGGTTGATGATAATCCTGATTTTACAATAGAAAAAATGCTGCGGGGCGATGAGGGATATCGCGAGAAATGGTATCTTGATTCAGAAGGATATCCGACAATAGGCATCGGGCATTTGATCATTTATAAGAAAACCAGTGATCTTGGTATTATCAATAATGAACTTTCTAAGCTGGTAGGGCGTGAAGTTACAAACGGAAGACTTACCGCCGAAGAAGTTAGTAAAGTATTTGCCGACGATATAGAAAAGACTCGTCGTGATATGCGCAAACATCCACGAATTGCACCTGTCTATAATAAATGTAATGCCTCTCGTCGTATGGCATTAGAAAATATGGCGTTTCAAATGGGTGTTGGTGGATTGGGTAAATTTAAGAATAGTCTTGCTGCGATGTTAGCGGAAGAATGGAAACAAGCCTATGATGGATTACGTCAATCGGTATGGGCTAACCAAACTCCAGGACGCTCCAGTCGTGTTTCTAAAATTATTTTAACTGGTAACTTAGAATCATATGGCGTAATTGCTCCAAAGAAAGAGGAATCAATTTCTGATGATCCGCGAGCACGTTTAAGAAATGCCCGTATTGCTGCCTATAAGACGCAGAGAGCAGAAGAAGACCCAGAAGCACCGTTTACCCCACAAGACACACGAATCATGTTTAAAGAGCCTAAGAGTTCGTATTCTGCGAGGTATCCTTATAACCATGTATACGAGTCAGAGAGCGGTCATATTATCGAGATTGATGATACTCCTTCTCATGAGCGATATCATCGTAAACATCCTTCTGGTACATTTGAAGAAACCAGACCGGACGGAACGAGGGTAGAAAAGATTGAAGGCGATGATTATCTGATCGTTAAACAAGGCCGGAAAGTAAACGTCAAGGGTAATTTGCAAGTTGTTGTTGAAGGTGACGCGCAAGTTTATTATATGGGTAATGTGATGCAAACCGTTGATGGTAACGTCACTGAATTTATTCGTGGCAATGTTAATCAGACCGTTGAAGGTACTGCTAACATGCATGTTGTAGGTGATGTTACCGCTCAGCTTGACGCTAATTTAACGGCTAATGTCAAGGGTAATGCTGAATTAATGATCGAGGGTAACACTACCGAAACAATTAAAGGTAACTATGATTTAACCGTTGAGGGTAATTTTAATATGACCGTCAATGGCACTAAATCAGACCAGGTTAATGGTGACTGGTCCCGTAATTGTGGTGCAAGCGTTAAAGATATTGCATCTGGTACAGTTAAAATTGATGGTAGCCGTATTGATCTTGGGTGA

Gene Ontology

	Description	Category	Evidence (source)
GO:0003796	lysozyme activity	Molecular function	Inferred from Electronic Annotation (UniProt)
GO:0009253	peptidoglycan catabolic process	Biological process	Inferred from Electronic Annotation (InterPro)
GO:0016998	cell wall macromolecule catabolic process	Biological process	Inferred from Electronic Annotation (InterPro)
GO:0031640	killing of cells of another organism	Biological process	Inferred from Electronic Annotation (UniProt)
GO:0042742	defense response to bacterium	Biological process	Inferred from Electronic Annotation (UniProt)
GO:0098003	viral tail assembly	Biological process	Inferred from Electronic Annotation (UniProt)
GO:0098025	virus tail, baseplate	Cellular component	Inferred from Electronic Annotation (UniProt)
GO:0098932	symbiont entry into host cell via disruption of host cell wall peptidoglycan	Biological process	Inferred from Electronic Annotation (UniProt)
GO:0098994	symbiont entry into host cell via disruption of host cell envelope	Biological process	Inferred from Electronic Annotation (UniProt)

Enzymatic activity

EC Number	Entry Name	Reaction Catalyzed	Classification	Evidence	Source
3.2.1.17	lysozyme aka muramidase	D-glucosamine residues in chitodextrins	Hydrolases Glycosylases Glycosidases, i.e. enzymes hydrolyzing O- and S-glycosyl compounds	match to sequence model evidence used in automatic assertion ECO:0000256	HAMAP-Rule:MF_04151

Tertiary structure

PDB ID: upi00015c1246_model

Method: AlphaFold3 Non-commercial

Resolution: –

Chain position: A