Protein

UniProt accession
K4F7R7 [UniProt]
Protein name
Pre-baseplate central spike protein
PhaLP type
endolysin

evidence: UniProt function annotation

probability: 99 % (predicted by ML model)

Protein sequence
MKFGGNMHSPFFEGVVENVDDPRLLGRVQVRVFGEHPAQKQKSETIGIPVEELPWMMPIQDIRSAAISGVGFSPTGITRGSFVIGYWRDKWHQDGVILGTFAGEYREKPDTQKGFCDPFGEYPRYVGNDVNVLARGGAEGRGSSSVTIRDANSSLAVNPDDRPLDEIPEDNRPDTGGFTIEKMIRGDEGIRLTWYYDVKGYTIGIGHFFLTAPQGTDPAVVNAALSRQIGRTVTGVPGSITADEASVLFQQDLAKVHNDIQNNSKVREVYVTLNRPRQMAIENMCFQMGVGGVAKFTNALAAMKRQDWQTAYNELRNSTWANQTPGRSSRVSKIVLTGNLESYGVQVPDPEGRSLSAAYNAVVLQASNPEDPFVPGDTRVMFEEPKSAYAGEYPYNMVFESRSGHIQEFDDTPGFERYNRVHPAGSYEEIRPDGTRVVKIVGDDYQIVMQGRKLNVKGNLQVVIEGDAFIYNMGNVQQTVDGNVTEFVRGNVNQTVEGEYVGLIKGNAELTVEKDATVNVNQNLMANVKQNATVAVTEDATITAKNALIDIEQDLDIGARNINMRASNNTFIDSGALTKITGGTVQVG
Physico‐chemical
properties
protein length:588 AA
molecular weight:64528,00000 Da
isoelectric point:5,05335
aromaticity:0,08163
hydropathy:-0,40935

Domains

Domains [InterPro]
IPR023346
131–347
IPR023346 IPR010609
IPR046397
4–588
IPR046397
IPR002196
188–322
IPR002196 IPR009590
IPR052619
179–342
IPR052619
IPR001165
277–296
IPR001165
IPR023347
178–346
IPR023347
Protein sequence: K4F7R7
1 588
Legend: Pfam SMART CDD TIGRFAM HAMAP SUPFAM PRINTS Gene3D PANTHER Other

Taxonomy

  Name Taxonomy ID Lineage
Phage Cronobacter phage vB_CsaM_GAP161
[NCBI] 		1141138 Straboviridae > Pseudotevenvirus > Pseudotevenvirus gap161
Host Cronobacter sakazakii
[NCBI] 		28141 Bacteria > Proteobacteria > Gammaproteobacteria > Enterobacteriales > Enterobacteriaceae > Cronobacter

Coding sequence (CDS)

Coding sequence (CDS)
Genbank protein accession
AFC22298.1 [NCBI]
Genbank nucleotide accession
JN882287 [NCBI]
CDS location
range 104609 -> 106375
strand +
CDS
ATGAAATTCGGTGGAAATATGCATTCCCCTTTCTTTGAGGGGGTGGTCGAAAACGTTGATGATCCTCGTCTACTGGGGCGTGTTCAGGTGCGTGTATTTGGTGAGCATCCGGCACAGAAACAAAAATCTGAAACTATCGGTATTCCGGTTGAGGAATTGCCGTGGATGATGCCTATTCAGGATATTCGCAGCGCCGCTATTTCCGGTGTTGGTTTTTCTCCGACTGGTATCACGCGCGGTAGTTTCGTTATCGGTTACTGGCGCGATAAATGGCACCAGGACGGCGTTATACTGGGAACCTTTGCTGGTGAGTACCGAGAGAAACCGGACACCCAGAAAGGCTTCTGTGACCCGTTTGGTGAGTATCCTCGTTATGTGGGTAACGATGTTAACGTTCTGGCTCGCGGTGGCGCAGAAGGGCGCGGTTCTTCGAGCGTCACTATCCGTGATGCAAACAGTTCATTAGCAGTCAACCCAGACGATCGCCCATTGGACGAGATTCCAGAAGATAACCGACCTGATACTGGTGGGTTTACGATAGAAAAAATGATCCGTGGTGATGAAGGTATTAGATTGACGTGGTATTATGATGTTAAAGGTTATACCATCGGGATCGGACACTTTTTCTTGACCGCACCACAAGGAACAGACCCAGCAGTTGTTAACGCTGCGTTATCTAGACAAATTGGCAGAACGGTTACTGGTGTTCCTGGATCAATCACTGCTGATGAAGCGAGTGTTTTATTTCAGCAGGATTTAGCTAAAGTTCATAACGATATTCAGAATAATAGCAAAGTTCGGGAAGTGTATGTGACGTTAAACCGTCCTCGTCAAATGGCTATTGAAAATATGTGCTTCCAAATGGGCGTTGGCGGTGTTGCCAAATTTACTAATGCTCTGGCTGCTATGAAGCGTCAGGACTGGCAAACCGCTTACAACGAACTGCGAAACTCTACATGGGCTAATCAGACGCCAGGACGTTCTTCACGCGTATCTAAAATCGTGCTGACGGGCAACCTTGAATCATACGGTGTTCAGGTTCCAGATCCAGAAGGGCGTTCATTGAGTGCTGCTTATAATGCGGTGGTTCTGCAAGCAAGCAACCCAGAAGATCCTTTTGTTCCTGGCGATACCCGCGTAATGTTCGAAGAACCTAAATCGGCATATGCTGGTGAATATCCATACAACATGGTATTCGAATCCCGTTCTGGACACATTCAGGAATTTGACGATACACCAGGCTTTGAGCGTTACAACCGCGTTCACCCAGCAGGATCATACGAAGAAATTCGTCCTGATGGAACCCGTGTTGTTAAAATCGTTGGTGATGATTATCAGATCGTAATGCAAGGCCGTAAATTAAACGTCAAGGGTAATTTACAGGTCGTTATCGAAGGTGACGCATTCATTTACAATATGGGTAACGTTCAACAGACCGTTGATGGTAACGTGACCGAGTTCGTGCGCGGTAACGTGAATCAAACCGTTGAAGGTGAATATGTGGGGCTGATTAAAGGCAACGCAGAATTAACCGTTGAAAAAGATGCAACCGTAAACGTGAATCAGAATTTAATGGCTAACGTTAAACAGAATGCAACCGTTGCCGTAACTGAGGATGCAACAATTACCGCAAAAAATGCTCTGATTGATATTGAGCAAGATTTGGATATTGGCGCACGTAATATCAACATGCGAGCATCGAATAATACTTTCATTGATTCCGGCGCACTGACTAAGATCACTGGCGGAACAGTCCAGGTAGGTTAA

Gene Ontology

Description Category Evidence (source)
GO:0003796 lysozyme activity Molecular function Inferred from Electronic Annotation (UniProt)
GO:0009253 peptidoglycan catabolic process Biological process Inferred from Electronic Annotation (InterPro)
GO:0016998 cell wall macromolecule catabolic process Biological process Inferred from Electronic Annotation (InterPro)
GO:0031640 killing of cells of another organism Biological process Inferred from Electronic Annotation (UniProt)
GO:0042742 defense response to bacterium Biological process Inferred from Electronic Annotation (UniProt)
GO:0098003 viral tail assembly Biological process Inferred from Electronic Annotation (UniProt)
GO:0098025 virus tail, baseplate Cellular component Inferred from Electronic Annotation (UniProt)
GO:0098932 symbiont entry into host cell via disruption of host cell wall peptidoglycan Biological process Inferred from Electronic Annotation (UniProt)
GO:0098994 symbiont entry into host cell via disruption of host cell envelope Biological process Inferred from Electronic Annotation (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.2.1.17 lysozyme
aka muramidase 		D-glucosamine residues in chitodextrins
Hydrolases
Glycosylases
Glycosidases, i.e. enzymes hydrolyzing O- and S-glycosyl compounds
 match to sequence model evidence used in automatic assertion
ECO:0000256 		HAMAP-Rule:MF_04151

Tertiary structure

PDB ID: upi00028ad6d3_model

Method: AlphaFold3 Non-commercial

Resolution: –

Chain position: A