Protein

UniProt accession

M1EB84 [UniProt]

Protein name

Baseplate central spike protein

PhaLP type

endolysin

evidence: UniProt function annotation

probability: 99 % (predicted by ML model)

Protein sequence

MDMINNNVEWFVGVVEDRMDPLKQGRVRVRVIGLHPFQKVQGPVSGMPTEDLPWMSVLQPITSASMSGIGGSVTGPVEGTHVYGHWLDKYRTNGLVMGTYGVNARVRPNTTEGFSDPTGQYPRYLGNDTNLLNQGGEAGFESIPNVIQDNNLDTAINPDDTDLSNIPEDNNPNYTIEAMLRRDEGLRLKVYWDTEGYPTIGIGHLIMRQRVRDMTQINKVLSNQVGREVKGNPGSISMDEASALFAKDLADMQRDIKTNSKVGPVYVKMNKSRQMALENMSFQMGVGGVANFTNMLKAMDEGRWKDAYNESRNSLWFQQTKGRASRVSMIILTGNMESYGVPVNPVQGKSVGAQLATVKASVNPSDPPIPSDSRILFKEPVSSYKGQYPYVHTMETESGHIQEFDDTPGYERYRLVHPTGTYEEVAPDGRRTRKTVNDLYDIANGDGNYLVSGDKKVNVGADETYYNMANRLHQIDGNNILFIRGDETKTVEGDGTLYVKGNIKIVVDGNADILVKGDAKTQVEGNHDYTVNGNVKWTVNGSVNMTVAGNWSETMSSMSSIAQGQYTIDGSRVDIGL

Physico‐chemical properties

protein length:	577 AA
molecular weight:	63714,00000 Da
isoelectric point:	5,31430
aromaticity:	0,07452
hydropathy:	-0,54125

Domains

Domains [InterPro]

IPR010609

533–555

IPR010609 IPR009590 IPR052619

IPR046397

3–576

IPR046397

IPR002196

183–311

IPR002196

IPR023347

178–342

IPR023347

IPR001165

177–196

IPR001165

IPR023346

131–341

IPR023346

Protein sequence: M1EB84

1 577

Legend: Pfam SMART CDD TIGRFAM HAMAP SUPFAM PRINTS Gene3D PANTHER Other

Domains [InterPro]

InterPro ID	Start	End	Source	Name
IPR010609	533	555	Pfam	Gp5, C-terminal
IPR046397	3	576	HAMAP	Pre-baseplate central spike protein Gp5
IPR009590	33	173	Pfam	Protein Gp5, N-terminal OB-fold domain
IPR052619	173	338	PANTHER	Bacteriophage lysozyme-like
IPR002196	183	311	Pfam	Glycoside hydrolase, family 24
IPR023347	178	342	Gene3D	Lysozyme domain superfamily
IPR001165	177	196	PRINTS	T4-type lysozyme
IPR023346	131	341	SUPFAM	Lysozyme-like domain superfamily

Taxonomy

	Name	Taxonomy ID	Lineage
Phage	Salmonella phage S16 (Salmonella phage vB_SenM-S16) [NCBI]	1087482	Straboviridae > Gelderlandvirus > Gelderlandvirus s16
Host	Salmonella enterica [NCBI]	28901	Bacteria > Proteobacteria > Gammaproteobacteria > Enterobacteriales > Enterobacteriaceae > Salmonella

Coding sequence (CDS)

Genbank protein accession

AEO97088.1 [NCBI]

Genbank nucleotide accession

HQ331142 [NCBI]

CDS location

range 67750 -> 69483
strand +

CDS

ATGGATATGATTAATAACAACGTCGAATGGTTTGTCGGCGTTGTAGAAGATAGAATGGACCCGTTAAAACAAGGACGAGTTCGTGTGAGAGTTATAGGTCTTCATCCATTCCAGAAAGTTCAAGGCCCTGTATCGGGAATGCCAACAGAAGACCTTCCATGGATGAGTGTTTTACAACCTATAACATCCGCATCTATGTCAGGAATAGGCGGTTCTGTCACTGGACCCGTCGAAGGAACTCATGTTTATGGTCATTGGTTGGACAAATATCGTACTAATGGTTTAGTGATGGGCACATACGGAGTAAATGCCAGAGTTAGACCAAACACTACAGAGGGTTTCTCTGATCCAACTGGCCAATATCCGCGTTACTTAGGTAATGATACTAACCTTTTGAACCAAGGCGGCGAAGCGGGATTTGAGTCAATCCCTAACGTTATTCAAGATAATAACCTTGATACAGCTATTAATCCCGACGACACAGATTTATCAAATATTCCAGAAGATAATAATCCAAATTATACTATAGAAGCTATGCTTCGCCGCGACGAAGGTCTTCGTTTGAAAGTATACTGGGACACTGAAGGATACCCGACTATCGGTATTGGGCATCTTATCATGAGACAAAGAGTCCGTGATATGACTCAAATTAATAAAGTGCTTTCAAATCAAGTTGGAAGAGAAGTTAAAGGAAATCCCGGTTCTATATCAATGGATGAAGCATCCGCATTATTTGCTAAAGACCTAGCTGATATGCAAAGAGATATAAAAACCAACTCAAAAGTCGGTCCAGTTTATGTCAAAATGAATAAATCGAGACAAATGGCTCTTGAAAATATGAGTTTTCAAATGGGAGTTGGTGGTGTAGCTAATTTCACTAATATGCTTAAAGCCATGGATGAAGGCCGTTGGAAAGATGCATATAACGAATCTCGAAACTCTTTATGGTTTCAGCAGACTAAAGGACGAGCTTCTAGAGTATCTATGATTATTCTTACTGGTAATATGGAATCGTACGGTGTTCCTGTTAATCCGGTTCAAGGAAAATCTGTTGGTGCTCAATTAGCTACTGTTAAAGCTTCAGTTAATCCAAGCGACCCTCCTATACCAAGTGACAGCAGAATTCTTTTTAAAGAGCCTGTATCATCATATAAAGGACAATACCCTTATGTCCATACAATGGAAACTGAATCTGGTCACATTCAAGAATTTGATGATACTCCGGGTTATGAAAGATATCGTCTTGTTCACCCAACAGGGACATATGAAGAAGTAGCTCCTGATGGAAGAAGAACTAGAAAAACAGTAAATGATTTATACGATATTGCTAATGGTGATGGAAATTATTTAGTTTCCGGTGATAAAAAGGTAAACGTTGGTGCAGATGAAACATACTATAACATGGCCAACAGATTACATCAAATTGACGGAAATAATATTTTGTTTATCCGTGGAGATGAAACAAAAACTGTCGAAGGAGATGGAACACTTTACGTTAAAGGAAATATAAAGATAGTCGTTGACGGAAATGCTGATATCTTGGTTAAAGGAGATGCTAAAACTCAGGTTGAAGGAAATCACGATTATACAGTAAACGGAAACGTAAAATGGACTGTAAATGGTAGTGTTAATATGACAGTTGCTGGGAATTGGTCAGAAACTATGAGCTCAATGTCTTCTATAGCTCAGGGCCAATATACTATTGATGGCAGTCGTGTGGACATTGGTTTATAA

Gene Ontology

	Description	Category	Evidence (source)
GO:0003796	lysozyme activity	Molecular function	Inferred from Electronic Annotation (UniProt)
GO:0009253	peptidoglycan catabolic process	Biological process	Inferred from Electronic Annotation (InterPro)
GO:0016998	cell wall macromolecule catabolic process	Biological process	Inferred from Electronic Annotation (InterPro)
GO:0031640	killing of cells of another organism	Biological process	Inferred from Electronic Annotation (UniProt)
GO:0042742	defense response to bacterium	Biological process	Inferred from Electronic Annotation (UniProt)
GO:0098003	viral tail assembly	Biological process	Inferred from Electronic Annotation (UniProt)
GO:0098025	virus tail, baseplate	Cellular component	Inferred from Electronic Annotation (UniProt)
GO:0098932	symbiont entry into host cell via disruption of host cell wall peptidoglycan	Biological process	Inferred from Electronic Annotation (UniProt)
GO:0098994	symbiont entry into host cell via disruption of host cell envelope	Biological process	Inferred from Electronic Annotation (UniProt)

Enzymatic activity

EC Number	Entry Name	Reaction Catalyzed	Classification	Evidence	Source
3.2.1.17	lysozyme aka muramidase	D-glucosamine residues in chitodextrins	Hydrolases Glycosylases Glycosidases, i.e. enzymes hydrolyzing O- and S-glycosyl compounds	match to sequence model evidence used in automatic assertion ECO:0000256	HAMAP-Rule:MF_04151

Tertiary structure

PDB ID: upi0002b28b23_model

Method: AlphaFold3 Non-commercial

Resolution: –

Chain position: A